62325 Microbiology 2 
Mike's lectures and laboratory sessions

 
 
 
 
 
 

written by Dr Mike Kotiw, 
Department of Biological & Physical Sciences, Faculty of Sciences
last modified April, 2000
Welcome to 62325 Microbiology 2 ONLINE. These webpages have been developed to assist you in the study of the material provided in my lectures.  Links to additional information and online access to the 8 lectures series are provided in the table below. Simply place the cursor onto the preferred option and click to access the material.
 
Mike's lectures and laboratory schedule
 Lecture Topic Practicals References
1-2 The Chlamydia
The Neisseria
The Spirochaetes		 Sherris
p 245-246
3
 

4

 Brief review of virus structure, modes 
of replication and classification

The DNA viruses: The herpesviruses

 S1: Prelab principles of tissue culture + video
examine 

S2: Examine SF9 cells and passage

 Sherris
p69-104

p503-518
5

6

 The DNA viruses: Hepatitis B virus

The RNA Viruses: HIV

 S3: Infect and transfect SF9 cells with wtBV and wtBVDNA
passage control SF9cells

S4: Examine cells for CPE, passage control SF9 cells

 Sherris
p491-502

p541-556
7

8

 The RNA viruses: HCV

The RNA viruses: RSV

 S5: Prepare SF9 cells for PCR from infection, transfection and control SF9 cells. perform PCR

S6: Analyse results- write up prac
 

 Sherris
p491-502
 

p451-466
Mike's Theory Examination Practice questions Mikes Prac exam practice questions

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Expectations:
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Written by  Mike Kotiw  modified February, 2000