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BIO2107 Cell and Molecular Biology 1

Semester 1, 2013 On-campus Toowoomba
Units : 1
Faculty or Section : Faculty of Sciences
School or Department : Biological & Physical Sciences
Version produced : 21 July 2014

Contents on this page

Staffing

Examiner: Mark Sutherland
Moderator: John Dearnaley

Requisites

Pre-requisite: CHE2120

Rationale

An understanding of the theory and techniques of cell and molecular biology are becoming an essential component in the research and medical diagnostic spheres. The course will provide the student with theoretical knowledge in basic molecular biology and cell biology. During the course students will gain an insight into the nature of cellular substructure, vesicle trafficking, protein targeting and control of the cell cycle. The students will also explore the nature of cells at the molecular level as well as the current molecular technologies that enable such information to be derived. Finally the students will gain knowledge of the nature of the molecular and other technologies that have advanced the analytical options in a pathology diagnostic laboratory.

Synopsis

The course is presented in two modules: in the first, the course introduces the nature of gene organisation, replication and expression in both prokaryotic and eukaryotic systems. The course also provides an overview of molecular technologies including recombinant DNA techniques and standard molecular methods such as Western Blot assays, PCR, rtPCR and real time PCR. These procedures underpin state of the art research and medical diagnostic assays. Applications of this technology are discussed. In the second module the course explores the nature of cellular ultrastructure; protein post-translational modification and targeting; vesicle transport in cells; regulation of the cell cycle and apoptosis.

Objectives

On completion of this course students will be able to:

  1. demonstrate an understanding of the current concepts of DNA structure maintenance and repair;
  2. explain the basic processes involved in gene replication, transcription and translation in both prokaryotic and eukaryotic systems;
  3. demonstrate an understanding of basic tools used in recombinant DNA technology including: enzymes, plasmids and techniques for cloning and characterisation;
  4. demonstrate the practical use of a range of basic molecular techniques including Western Blot, PCR, rtPCR and real time PCR;
  5. demonstrate a basic understanding of post-translational protein modification;
  6. demonstrate an understanding of the cytoskeleton and the structure, function and maintenance of cell organelles;
  7. demonstrate an understanding of cellular trafficking, cell cycle control mechanisms and apoptosis.

Topics

Description Weighting(%)
1. Genome organisation in prokaryotic and eukaryotic cells 4.00
2. DNA replication and repair 8.00
3. Transcription and translation 8.00
4. Gene expression in prokaryotes 8.00
5. Gene expression in eukaryotes 8.00
6. Recombinant DNA techniques 10.00
7. Molecular application is a medical pathology laboratory 10.00
8. Postranslational protein modification 8.00
9. Protein sorting and targeting 8.00
10. Intracellular compartmentalisation 10.00
11. Cellular cytoskeleton 8.00
12. Cell cycle control and apoptosis 10.00

Text and materials required to be purchased or accessed

ALL textbooks and materials available to be purchased can be sourced from USQ's Online Bookshop (unless otherwise stated). (https://bookshop.usq.edu.au/bookweb/subject.cgi?year=2013&sem=01&subject1=BIO2107)

Please contact us for alternative purchase options from USQ Bookshop. (https://bookshop.usq.edu.au/contact/)

  • Karp, G 2010, Cell and molecular biology, 6th edn, Wiley, New York.
    (ISBN 9780470483374.)

Reference materials

Reference materials are materials that, if accessed by students, may improve their knowledge and understanding of the material in the course and enrich their learning experience.
  • Alberts, B et al 2010, Essential cell biology, 3rd edn, Garland Science, New York.
  • Bolsover, SR, Hyams, JS, Shephard, EA, White, HA & Wiedmann, CG 2004, Cell biology a short course, 2nd edn, John Wiley & Sons, New Jersey.
    (ISBN 0 471 26393 1.)
  • Brown, TA (ed.) 2000, Essential molecular biology: A practical approach, 2nd edn, IRL Press, Oxford.
  • Lewin, B, Cassimeris, L, Lingappa, VR & Plopper, G 2007, Cells, Jones & Bartlett Publishers, Massachusetts.
    (ISBN 0 7637 3905 9.)
  • Nelson, DL & Cox, MM 2008, Lehninger principles of biochemistry, 5th edn, WH Freeman, New York.
  • Nicholl, D 2002, Introduction to genetic engineering, Cambridge University Press, Cambridge.
    (ISBN 0-521- 43054-2HC.)
  • Weissensteiner, T, Griffin, HG & Griffin, A 2004, PCR technology: Current innovations, CRC Press, Boca Raton, Fla.
    (0-8493-1184-5.)
  • Wu, W, Welsh, MJ & Zhang, HH 2004, Gene biotechnology, 2nd edn, CRC Press, Boca Raton, Fla.
    (ISBN 0-8493-1288-4.)

Student workload requirements

Activity Hours
Assignments 24.00
Examinations 2.00
Online Lectures 26.00
Private Study 90.00
Tutorials 26.00

Assessment details

Description Marks out of Wtg (%) Due Date Notes
Assignments 60 40 26 Feb 2013 (see note 1)
2Hr Restricted Exam 120 60 End S1 (see note 2)

NOTES
  1. Examiner will advise due dates for assignments.
  2. Examination dates will be available during the Semester. Please refer to the examination timetable when published.

Important assessment information

  1. Attendance requirements:
    It is the students' responsibility to attend and participate appropriately in all activities (such as lectures, tutorials, laboratories and practical work) scheduled for them, and to study all material provided to them or required to be accessed by them to maximise their chance of meeting the objectives of the course and to be informed of course-related activities and administration. To maximize their chances of satisfying the objectives of the practical component of the course, students should attend and actively participate in the laboratory sessions in the course.

  2. Requirements for students to complete each assessment item satisfactorily:
    To complete the assignment satisfactorily, students must obtain at least 50% of the marks available for the assignment. To complete the examination and test satisfactorily, students must obtain at least 50% of the marks available for the examination and test.

  3. Penalties for late submission of required work:
    If students submit assignments after the due date without (prior) approval of the examiner then a penalty of 5% of the total marks gained by the student for the assignment may apply for each working day late up to ten working days at which time a mark of zero may be recorded.

  4. Requirements for student to be awarded a passing grade in the course:
    To be assured of receiving a passing grade a student must achieve at least 50% of the total weighted marks available for the course.

  5. Method used to combine assessment results to attain final grade:
    The final grades for students will be assigned on the basis of the weighted aggregate of the marks (or grades) obtained for each of the summative assessment items in the course.

  6. Examination information:
    Candidates are allowed access only to specific materials during a Restricted Examination. The only materials that candidates may use in the restricted examination for this course are: calculators which cannot hold textual information.

  7. Examination period when Deferred/Supplementary examinations will be held:
    Any Deferred or Supplementary examinations for this course will be held in the fourth week of the semester following this course offering and the examiner will advise students involved in writing of the date time and location of any such examination.

  8. University Student Policies:
    Students should read the USQ policies: Definitions, Assessment and Student Academic Misconduct to avoid actions which might contravene University policies and practices. These policies can be found at http://policy.usq.edu.au.

Assessment notes

  1. Students who, for medical, family/personal, or employment-related reasons, are unable to complete an assignment or to sit for an examination at the scheduled time may apply to defer an assessment in a course. Such a request must be accompanied by appropriate supporting documentation. One of the following temporary grades may be awarded IDS (Incomplete-Deferred Examination); IDM (Incomplete Deferred Make-up); IDB (Incomplete - Both Deferred Examination and Deferred Make-up).

  2. The due date for an assignment is the date by which a student must despatch the assignment to the USQ. The onus is on the student to provide proof of the despatch date, if requested by the Examiner. Students must retain a copy of each item submitted for assessment. If requested by the Examiner, students will be required to provide a copy of assignments submitted for assessment purposes. Such copies should be despatched to USQ within 24 hours of receipt of a request being made. The examiner of a course may grant an extension of the due date of an assignment in extenuating circumstances. The Faculty will normally only accept assessments that have been written, typed or printed on paper-based media. The Faculty will NOT accept submission of assignments by facisimile. Students who do not have regular access to postal services or who are otherwise disadvantaged by these regulations may be given special consideration. They should contact the examiner of the course to negotiate such special arrangements. In the event that a due date for an assignment falls on a local public holiday in their area, such as a Show holiday, the due date for the assignment will be the next day. Students are to note on the assignment cover the date of the public holiday for the Examiner's convenience.

  3. Harvard (AGPS) is the referencing system required in this course. Students should use Harvard (AGPS) style in their assignments to format details of the information sources they have cited in their work. The Harvard (AGPS) style to be used is defined by the USQ Library's referencing guide. http://www.usq.edu.au/library/referencing