62325 MICROBIOLOGY 2

Year	No.	Offer	Mode	Description			Cred. Pts
00	62325 	S1  	D 	MICROBIOLOGY 2            	1.00

Contents

STAFFING:

Examiner: T. MUKKUR
Moderator: M. KOTIW

PRE-REQUISITE(S)

62215

RATIONALE:


This  course  covers the mechanisms of pathogenesis of  medically  and
veterinarily  significant micro-organisms. Also  covered  are  various
facets of the immune system including molecular and cellular basis  of
the immune response to self and non-self antigens including infectious
disease  agents,  autoimmunity,  immunogenetics,  transplantation  and
cancer  immunology. The aim of this course is to provide  the  student
with  additional  alternative  possible  career  options  within   the
discipline of biotechnology and cell and molecular biology.

SYNOPSIS:


This unit emphasises the mechanisms of microbial pathogenesis and  the
approaches  taken for prevention of significant microbial diseases  of
animals and humans. The diseases covered include those caused by gram-
positive,   gram-negative  and  acid-fast   micro-organisms   and   an
introduction  to  significant  fungal  and  protozoal  diseases.  Also
considered  are  the concepts of antigenic specificity,  induction  of
humoral   and  cellular  immune  response,  immunoglobulin  structure,
function  and  genetic basis of antibody diversity, the  role  of  the
immune  system in health and disease including cell-mediated immunity,
hypersensitivity reactions, autoimmunity, and cancer immunology.

OBJECTIVES:

On successful completion of this unit students will be able
to:

  1. relate the genus and species of the microorganism with the
    disease syndrome of animals and humans;
  2. summarise the medically and veterinarily important members of
    the normal flora and their anatomic locations;
  3. describe the determinants of pathogenesis of important
    organisms;
  4. describe the applications of molecular biological techniques
    particularly DNA fingerprinting in the diagnosis of disease-
    causing microorganisms;
  5. describe the currently available means of prevention of
    significant diseases of animals and humans;
  6. describe the modes of transmission and symptoms of diseases
    caused by medically important organisms;
  7. differentiate between medically significant organisms causing
    disease in humans;
  8. name opportunistic pathogens and their role in disease
    processes;
  9. describe the nonspecific defenses which protect against
    microorganisms in general;
  10. describe different classes/subclasses/isotypes of immuno-
    globulins / antibodies, their structure and functions;
  11. describe components of the complement system and its function
    in host defense;
  12. describe the chemical basis of antigenic specificity;
  13. describe the current concepts in the generation of humoral and
    cellular immune response;
  14. describe different types of hypersensitivities and their role
    in health and disease;
  15. describe the phenomenon of autoimmunity as a disease process;
  16. describe the current concepts in vaccine development and their
    success in today's world;
  17. describe the immunological basis for rejection/acceptance of
    cell/organ transplants;
  18. describe the current state of achievements in the field of
    cancer immunology;
  19. purify immunoglobulins from serum using affinity
    chromatography;
  20. perform basic serological tests used to diagnose infectious
    diseases eg. agglutination, precipitation,
    immunoeletrophoresis and enzyme-linked immunosorbent assays;
  21. perform western blotting to enable identification of
    significant antigens.

TOPICS:

 Description                                                    Weighting(%)
    

  1. Cellular  and Molecular events underpinning  outcomes  in
human or animal-pathogen interactions in:
     A.   Foodborne and waterborne bacterial infections   10
     B.   Airborne bacterial infections                   10
     C.   Sexually transmitted bacterial infections       7
     D.   Nosocomial bacterial infections                 3
     E.   Significant viral infections                    8
     F.   Significant protozoal and fungal infections     5

    

  2. Molecular and Cellular Basis of Immune Response.
    (1)  Antigens/Haptens:Serological basis of
         specificity                                      5
    (2)  Generation of humoral and cellular
         immune response                                  8
    (3)  Complement System, Immunoglobulin Structure
         and function                                     8
    (4)  Molecular basis of antibody diversity            5
    (5)  Hypersensitivity immunological reactions         8
    (6)  Autoimmunity vs. tolerance                       8
    (7)  Oncogenes and cancer immunology                  8
    (8)  Microbial and plant biotechnologies in vaccine
         development                                      7

TEXT and MATERIALS to be PURCHASED:

Benjamini, et al 1996, Immunology : A Short Course, Wylie-Liss.

Practical Manual for Microbiology 2, Immunology and Biomedical
Microbiology, T K S MUKKUR, U.S.Q.

Ryan, K.J. 1994, Sherris Medical Microbiology, Appleton & Lange.

RECOMMENDED REFERENCE MATERIALS:

Alberts', B., Bray, D., Lewis, J., Raff, M., Roberts, K., & Watson,
J.D. 1994, The Molecular Biology of the Cell, 3rd edn., Garland, NY.

Banwart, G.J. 1996, Basic Food Microbiology, 2nd edn, Van Nostrand,
Rheinhold, NY.

Baron, 1994, Medical Microbiology, Jacaranda-Wiley.

Brown, F. 1994, Recombinant Vectors in Vaccine Development, Karg,
(DA Books).

Brown, F., Dougan, G., Hoey, E.M., et al, 1993, Vaccine Design,
Jacaranda-Wiley.

Coleman, R.M., Lombard, M.F. & Sicord, R.E. 1992, Fundamental
Immunology, 2nd edn, Mosby-Williams and Wilkins Pty. Ltd.

Davis, B.D., Dulbecco, Renato et al, 1990, Microbiology, 4th edn,
J.B. Lippincott Company.

Gardner, J.F. & Peel, M.M. 1991, Introduction to Sterilization,
Disinfection & Infection Control, 2nd edn, Churchill Livingstone,
Melbourne.

Glazer, A.N. & Nikaido, H. 1995, Microbial Biotechnology;
Fundamentals of Applied Microbiology, W.H. Freeman and Company.

Glick, B.R. & Pasternak, J.J. 1994, Molecular Biotechnology;
Principles and Applications of Recombinant DNA, ASM Press,
Washington, D.C.

Holt, J.G. 1984, 86, 89 & 89, Bergey's Manual of Systematic
Bacteriology, Volumes 1, 2, 3 & 4 Williams Wilkins, Baltimore.

Jawetz, E., Melnick, J.L. & Adelberg, E.A. 1991, Review of Medical
Microbiology, 19th edn, Lange Medical, Los Altos, CA.

Koneman, E.W., Allen, S.J., et al, Diagnostic Microbiology, J.B.
Lippincott.

Kreier, J.P., & Mortensen, R.F. 1990, Infection, Resistance and
Immunity, Harper & Row, NY.

Liddell, J.E. 1991, A Practical Guide to Monoclonal Antibodies,
Jacaranda-Wiley.

Mandell et al 1995, Principles and Practice of Infectious Diseases,
Vol. 1 & 2, Churchill Livingstone.

Neidhardt, F.C., Ingraham, J.L. & Schaechter, M. 1990, Physiology of
the Bacterial Cell - A Molecular Approach, Sinaver Assoc. Inc.,
Sunderland, MA.

Peters, J. & Schulze, M. 1992, Monoclonal Antibodies:A Practical
Guide, S P R B (DA Books)

Roitt, I., Brostoff, J. & Male, D. 1993, Immunology, 3rd edn, Mosby.

Samelson, L.E. & ED. 1994, Lymphocyte Activation, Karg (DA Books)

Saylers, A.A. & Whitt, D.D. 1994, Bacterial Pathogenesis:A Molecular
Approach, Blackwell Science Pty. Ltd. Carlton South. Victoria.

Talwar, G.P., RAO, K. & Chauhan, V.S. 1994, Recombinant and Synthetic
Vaccines, Naro (DA Books).

Volk, W.A., Benjamin D. & C., Kadner, R.J. & Parsons, T.T. 1991,
Essentials of Medical Microbiology, 4th edn, J.B. Lippincott.

STUDENT WORKLOAD REQUIREMENTS:

	ACTIVITY				HOURS
Lectures                                      	28
Laboratory or Practical Classes               	56
Private Study                                 	47
Examinations                                  	3
Assessments                                   	36

ASSESSMENT DETAILS:

No  *F/S Marks     Due        Description                              Wtg(%)    LBL WWW
1   S              END S1    LAB TEST (2 HOURS)                        20.00     N   N
2   S              MID-SEM   MIDTERM TEST                              20.00     N   N
3   S              END SEM   PROBLEM SET (1)                           10.00     N   N
4   S              END S1    3 HOUR CLOSED EXAMINATION                 50.00     N   N

*F=Formative, S=Summative

OTHER REQUIREMENTS:

1    Safe procedure in the laboratory will be assessed regularly and a
     satisfactory level is mandatory for a pass in the unit.
2    In  accordance  with  University's  Assignment  Extension  Policy
     (Regulation  5.9), the examiner of a unit may grant an  extension
     of  the  due  date of an assignment in extenuating circumstances.
     This  policy  may  be  found in the USQ  Handbook,  the  Distance
     Education  Study  Guide and the Faculty of Sciences'  Orientation
     Handbook for new on-campus students.  All students are advised to
     study and follow the guidelines associated with this policy.
3    Closed Examination: a closed examination is an examination  where
     the  candidates  are  allowed to bring only writing  and  drawing
     instruments into the examination.
This information is accurate as at 31/10/00