Passenger transportation is an essential part of the regional tourism industry. In general, a recent development in public transportation has been the rapid growth of Real-Time Ridesharing (RTR). However, it is unclear what impact RTR services have on the economic, social and environmental sustainability of the places in which they are currently operating, including regional tourism destinations (RTDs). It will contribute a new conceptual framework for assessing the social, economic and environmental sustainability of RTR in RTDs more generally, as well as providing a new understanding from multiple local perspectives of how RTR is affecting the sustainability of RTDs. The investigation findings will provide empirical knowledge of how RTRs are functioning and affecting sustainability in RTDs that may further inform RTDs’ decision making.